聂黔丽，王修俊 ，张二康，刘林新，于 沛，杨丽平，陈颜红

（贵州大学酿酒与食品工程学院，贵州省发酵工程与生物制药重点实验室，贵州贵阳 550025）

摘要：试验利用从自然发酵泡菜中筛选出的一株性能优良的优势菌株乳杆菌LF-8001进行高密度培养，通过单因素试验和正交实验分析初始pH、接种量、培养温度、外加营养因子种类以及中和剂种类对乳杆菌LF-8001高密度细胞培养的影响。确定乳杆菌LF-8001的最佳高密度细胞培养工艺为：以2%的西葫芦汁液作为外加营养因子、0.5% K2HPO4溶液为中和剂，调节MRS培养基初始pH为7，接种6%的乳杆菌LF-8001后于30 ℃恒温培养至稳定期，在此条件下乳杆菌LF-8001的菌浓度达到了1.08×1010 CFU/mL。

关键词：发酵乳杆菌LF-8001；高密度培养

中图分类号：TS201.3   文献标识码：A   文章编号：1674-506X（2021）02-0073-0005

High Cell Density Culture of Lactobacillus Fermentum LF-8001

NIE Qian-li, WANG Xiu-jun, ZHANG Er-kang, LIU Lin-xin, YU Pei, YANG Li-ping, CHEN Yan-hong

（School of Liquor and Food Engineering, Guizhou Provincial Key Laboratory of Fermentation Engineering and Biopharmacy,Guizhou University, Guiyang Guizhou 550025, China）

Abstract：A superior strain of Lactobacillus LF-8001 was selected from the natural fermented pickles for high cell density cultivation. The effects of initial pH, inoculum volume, culture temperature, kinds of additional nutritional factors and neutralizers on LF-8001 high cell density cultivation were analyzed by single factor and orthogonal experiments. The optimal high cell density cultivation technology of LF-8001 was determined as follows: 2% zucchini juice was used as an additional nutrient factor, 0.5% K₂HPO₄ solution was used as neutralizing agent, the initial pH of MRS medium was adjusted to 7, and 6% LF-8001 was inoculated, and then cultured to stable phase at 30 ℃. Under these conditions, the bacterial concentration of LF-8001 reached 1.08×1010 CFU/mL.

Keywords：Lactobacillus fermentum LF-8001; high cell density cultivation

doi：10.3969/j.issn.1674-506X.2021.02-010

收稿日期：2020-08-06

基金项目：贵州省科技计划项目（黔科合成果［2020］1Y025）；贵州省科技计划项目（黔农函［2020］43号）；贵州省科技计划项目（黔科合支撑［2017］2555）；贵州省发酵工程与白酒酿造人才基地（黔人领发［2018］3号）

作者简介：聂黔丽（1996-），女，硕士研究生。研究方向：食品发酵与保藏。

引文格式：聂黔丽,王修俊,张二康,等.发酵乳杆菌LF-8001高密度细胞培养的研究[J].食品与发酵科技,2021,57(2):73-76,90.